ISO 3475:2020

ISO 3475:2020 pdf free.Essential oil of aniseed (Pimpinella anisum L.)
1 Scope
This document specifies certain characteristics of the essential oil of aniseed (Pimpinella anisum L.),
with a view to facilitating the assessment of its quality.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO/TS 210, Essential oils — General rules for packaging, conditioning and storage
ISO/TS 211, Essential oils — General rules for labelling and marking of containers
Iso 212, Essential oils — Sampling
Iso 279, Essential oils — Determination of relative density at 20 degrees C — Reference method
ISO 280, Essential oils — Determination of refractive index
ISO 592, Essential oils — Determination of optical rotation
ISO 875, Essential oils — Evaluation of miscibility in ethanol
ISO 1041, Essential oils — Determination offreezing point
ISO 11024 (all parts), Essential oils — General guidance on chromatographic profiles
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at
— IEC Electropedia: available at
3.1 essential oil of aniseed essential oil obtained by steam distillation of the fruits of Pimpinella anisum L., of the Apiaceae family Note 1 to entry: For information on CAS number, see ISO/TR 21092.
4 Requirements
4.1 General requirements
Essential oil of aniseed (Pimpinella anisum L.) shall meet the requirements as given in Table 1.
4.2 Chromatographic profile
Carry out the analysis of the essential oil by gas chromatography. Determine the chromatographic profile in accordance with the Iso 11024 series. Identify in the chromatogram obtained the representative and characteristic components shown in Table 2. The proportions of these components, indicated by the integrator, shall be as shown in Table 2. This constitutes the chromatographic profile of the essential oil.
5 Flashpoint
Information on the flashpoint is given in Annex B.
6 Sampling
Sampling shall be performed in accordance with ISO 212. The minimum volume of the test sample is 50 ml.
NOTE The volume allows each of the tests specified in this document to be carried out at least once.
7 Packaging, labelling, marking and storage
These items shall be in accordance with ISO/TS 210 and ISO/TS 211.
B.1 General information
For safety reasons, transport companies, insurance companies and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products.
A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that:
— there is a wide variation in the chemical composition of essential oils;
— the volume of the sample needed for certain test equipment is incompatible with the high price of essential oils;
— as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only.
Consequently, it was decided to give a mean value for the flashpoint annexed to each International
Standard, for information, in order to meet the requirements of the interested parties.
The equipment with which this value was obtained has to be specified.
For further information see ISO/TR 11018.
B12 Flashpoint of the essential oil of aniseed [Pimpinella anisum L.]
The mean value is +98 °C.
NOTE Obtained with Pensky-Martens closed-cup equipment.
ISO 3475 pdf download.

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